Negative Staining and Immune Electron Microscopy as Techniques for Rapid Diagnosis of Viral Agents
نویسندگان
چکیده
منابع مشابه
Electron microscopy in rapid viral diagnosis: an update.
Diagnostic electron microscopy (DEM) has conceptual predecessors â€" the application of the sense of vision and of light microscopy in medicine. The evolvement of DEM and the role of its two branches - histopathology and rapid negative-contrast DEM - are described in this review, with a focus on the latter. By its resolving power of 2 nm in praxi, DEM is able to visualize all kinds of pathogens...
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A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.
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BACKGROUND Negative-staining (NS), a rapid, simple and conventional technique of electron microscopy (EM), has been commonly used to initially study the morphology and structure of proteins for half a century. Certain NS protocols however can cause artifacts, especially for structurally flexible or lipid-related proteins, such as lipoproteins. Lipoproteins were often observed in the form of rou...
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Epon epoxy resin was introduced as an embedding medium for biological electron microscopy largely through the independent efforts of Finck (1) and Luft (2). The usual recipe involves three components: Epon 812 resin (Epikote 812, in England), an anhydride hardener, and an accelerator such as DMP-30. Since the combination of Epon with dodecenyl succinic anhydride (DDSA) was thought to produce a ...
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enzyme-linked immunosorbent assay and nucleic acid amplification tests. After a simple and fast negative stain preparation, the undirected, “open view” of electron microscopy allows rapid morphologic identification and differential diagnosis of different agents contained in the specimen. Details for efficient sample collection, preparation, and particle enrichment are given. Applications of dia...
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ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 1983
ISSN: 0077-8923,1749-6632
DOI: 10.1111/j.1749-6632.1983.tb22228.x