New approach to use ethidium bromide monoazide as an analytical tool

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Possible errors in the interpretation of ethidium bromide and PicoGreen DNA staining results from ethidium monoazide-treated DNA.

Nocker and Camper reported that DNA from dead bacterial cells (Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium) treated with ethidium monoazide (EMA) is lost during the DNA extraction procedure (2). This was concluded from the missing or less intense DNA band on an agarose gel stained with ethidium bromide in comparison to the intensities of bands in untreated samples. Acco...

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ژورنال

عنوان ژورنال: Journal of Applied Microbiology

سال: 2010

ISSN: 1364-5072

DOI: 10.1111/j.1365-2672.2010.04716.x