New Granada Medium for detection and identification of group B streptococci

Liquid Granada medium for detection of group B streptococci.

Heelan et al. (5) recently presented data on the sensitivity of a liquid Granada medium (9) (GBS broth) for detection of Streptococcus agalactiae. We have tested this medium, and it contains, as Granada medium, starch, proteose peptone 3, methotrexate, a Good’s buffer, serum, glucose, and selective agents. So, we agree with the authors that detection of orangered colonies in GBS broth is 100% s...

Evaluation of liquid biphasic Granada medium and instant liquid biphasic Granada medium for group B streptococcus detection.

INTRODUCTION Group B streptococci (GBS) are transmitted from the mother to the newborn. Prevention of neonatal infection is achieved by intrapartum prophylaxis given to mothers colonized with GBS at 35 to 37 weeks of pregnancy. MATERIALS AND METHODS Liquid biphasic Granada medium (LB) and instant liquid biphasic Granada medium (ILB) were evaluated for GBS detection. Vaginal swabs obtained fro...

New medium for enhancing pigment production of group B streptococci.

Selective broth medium for isolation of group B streptococci.

A selective medium containing Todd-Hewitt broth, sheep blood, nalidixic acid, and gentamicin sulfate was found to enhance significantly the isolation of group B streptococci from vaginal cultures. Preparation of the medium, which is stable for up to 4 weeks at 4 C, is simple and inexpensive. Use of such a medium should facilitate identification of vaginal colonization with group B streptococci.

Evaluation of the Granada agar plate for detection of vaginal and rectal group B streptococci in pregnant women.

Granada medium was evaluated for the detection of group B streptococci (GBS) in vaginal and rectal swabs compared with selective Columbia blood agar and selective Lim broth. From May 1996 to March 1998, 702 pregnant women (35 to 37 weeks of gestation) participated in this three-phase study; 103 (14.7%) of these women carried GBS. In the first phase of the experiment (n = 273 women), vaginorecta...