New QuEChERS method for quantification of Physalin B and D in Physalis angulata L. in Vietnam
نویسندگان
چکیده
In Vietnam, Physalis angulata L. has been widely used as a traditional medicine to treat fever, anti-inflammatory, and expectorant. Currently, there have studies on the content of chemical composition especially physalin with anti-diabetic, antibacterial, prevent cancer. This study developed reliable sensitive method determine validate simultaneous Physalin B D in L.. The QuEChERS was for sample preparation from leaf matrices quantified by using High-performance liquid chromatography coupled diode-array detector. research validated under AOAC ICH guidance. Chromatography conditions include Agilent C 18 column (250mm × 4,6mm; 5µm) gradient mode acetonitrile – methanol-water mobile phase. recovery ranged 94.21 105.93% RSD 1.20 2.31%, LOD, LOQ were 0.4 mg/kg 2.4 mg/kg, respectively. results showed that proposed new quantification Vietnam.
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ژورنال
عنوان ژورنال: ????????
سال: 2022
ISSN: ['2411-6076', '2709-135X']
DOI: https://doi.org/10.3897/pharmacia.69.e89580