Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach

Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted i...

متن کامل

GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis

Building expression constructs for transgenesis is one of the fundamental day-to-day tasks in modern biology. Traditionally it is based on a multitude of type II restriction endonucleases and T4 DNA ligase. Especially in case of long inserts and applications requiring high-throughput, this approach is limited by the number of available unique restriction sites and the need for designing individ...

متن کامل

A Modular Cloning System for Standardized Assembly of Multigene Constructs

The field of synthetic biology promises to revolutionize biotechnology through the design of organisms with novel phenotypes useful for medicine, agriculture and industry. However, a limiting factor is the ability of current methods to assemble complex DNA molecules encoding multiple genetic elements in various predefined arrangements. We present here a hierarchical modular cloning system that ...

متن کامل

TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for expression tests. In this study, the TA-GC cloning method is proposed, as a new, simple and efficien...

متن کامل

High-efficiency cloning system for versatile adaptation of DNA fragments.

Recombinant DNA vectors are fundamental tools in molecular biology and genetic engineering. One of the problems frequently encountered in vector construction is the lack of compatible restriction sites between the vectors and the DNA inserts. The conventional approach to solve this problem is to make the DNA fragments blunt-ended (3,4,12) or to add linkers or adaptors (10,11,14) before ligation...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Frontiers in Bioengineering and Biotechnology

سال: 2020

ISSN: 2296-4185

DOI: 10.3389/fbioe.2019.00460