Nonisotopic, PCR-Based Method for 5¢ Insulin Gene VNTR Allele Class Assignment
نویسندگان
چکیده
منابع مشابه
Nonisotopic, PCR-based method for 5' insulin gene VNTR allele class assignment.
The identification of genes that contribute to the susceptibility to polygenic human diseases is a laborious task that requires the analysis of multiple polymorphic markers in search of variants putatively associated with the disease phenotype. Besides, it is often necessary to study large cohorts of affected and unaffected individuals to achieve acceptable levels of significance. Once prelimin...
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In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a smal...
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On the basis of the near-complete linkage disequilibrium of the insulin variable number of tandem repeats (INS VNTR) allele with the neighboring -23Hph1 A/T single-nucleotide polymorphism, previous studies have documented the association of class I ("short") and class III ("long") INS VNTR alleles with metabolic parameters, including circulating insulin levels. Using a new method to sequence cl...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00295bm03