Notch3 Activation Is Sufficient but Not Required for Inducing Human T-Lineage Specification

Notch3 activation is sufficient but not required for inducing human T-lineage specification.

Although the role for the individual Notch receptors in early hematopoiesis have been thoroughly investigated in mouse, studies in human have been mostly limited to the use of pan-Notch inhibitors. However, such studies in human are important to predict potential side effects of specific Notch receptor blocking reagents because these are currently being considered as therapeutic tools to treat ...

Notch signaling activation in human embryonic stem cells is required for embryonic, but not trophoblastic, lineage commitment.

The Notch signaling pathway plays important roles in cell-fate determination during embryonic development and adult life. In this study, we focus on the role of Notch signaling in governing cell-fate choices in human embryonic stem cells (hESCs). Using genetic and pharmacological approaches, we achieved both blockade and conditional activation of Notch signaling in several hESC lines. We report...

Policy Capacity Is Necessary but Not Sufficient; Comment on “Health Reform Requires Policy Capacity”

Policy capacity focuses on the managerial and organizational abilities to inform policy decisions with sound research and analysis, and facilitate policy implementation with operational efficiency. It stems from a view of the policy process that is rational and positivistic, in which optimal policy choices can be identified, selected, and implemented with objectivity. By itself, however, policy...

JNK2 is required for efficient T-cell activation and apoptosis but not for normal lymphocyte development

BACKGROUND The Jun N-terminal kinase (JNK) signaling pathway has been implicated in cell proliferation and apoptosis, but its function seems to depend on the cell type and inducing signal. In T cells, JNK has been implicated in both antigen-induced activation and apoptosis. RESULTS We generated mice lacking the JNK2 isozymes. The mutant mice were healthy and fertile but defective in periphera...

Transplantation Tolerance Sufficient for Inducing Islet But Not Skin Blockade of CD40-Mediated Signaling Is