Novel method for selective isolation of actinomycetes

Novel method for selective isolation of actinomycetes.

A new technique for the selective isolation of actinomycetes from natural mixed microbial populations is described. A nutrient agar medium was overlaid with a 0.22- to 0.45-microns-pore cellulose ester membrane filter, and the surface of the filter was inoculated. During incubation, the branched mycelia of the actinomycetes penetrated the filter pores to the underlying agar medium, whereas grow...

Selective isolation of aerobic Actinomycetes.

The composition of an arginine-glycerol-salt medium (AGS), suitable for the selective isolation of aerobic actinomycetes, was given. When soil samples were treated with calcium carbonate and plated on the AGS medium, higher total and relative plate counts of actinomycetes were obtained than when other media and methods were used.

Selective Isolation of Rare Actinomycetes Producing Novel Antimicrobial Compounds

Microbial pathogens are developing resistance against existing antibiotics, stressing the urgency for discovery of new therapeutic compounds. Actinomycetes, alone produce 70-80% of the available antibiotics. The chances of isolating undiscovered strains from the terrestrial habitats have diminished so that the search for novel products has switched to rare genera of actinomycetes from normal ha...

Method for the preferential isolation of Actinomycetes from soils.

Improvement of an Isolation Medium for Actinomycetes

By comparing differences among the effects of several isolation mediums on soil actinomycetes, we attempted to find a isolation medium that could result in actionomycetes with high diversity. Soil samples were isolated by the dilution plateing method, and genomic DNA was extracted using phenol-chloroform method. After amplification by 16S rDNA PCR, PCR products were sequenced and undertaken phy...