NT*-HRV3CP: An optimized construct of human rhinovirus 14 3C protease for high-yield expression and fast affinity-tag cleavage
نویسندگان
چکیده
The human rhinovirus 14 3C protease (HRV3C protease), in fusion with glutathione S-transferase also referred to as PreScission™ protease, is a cysteine of particular interest for affinity tag removal from proteins due its stringent recognition sequence specificity (LEVLFQ/GX) and superior activity at low temperature. Here we report the expression, purification use construct HRV3C NT*-HRV3CP, that affords high expression yield E. coli (over 300 mg/L cell culture), facile single-step purification, solubility (>10 mg/mL) excellent storage properties. NT*-HRV3CP cleaves tags 4 °C minutes, making it an attractive tool production recombinant biotechnological, industrial pharmaceutical applications.
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2021
ISSN: ['1873-4863', '0168-1656']
DOI: https://doi.org/10.1016/j.jbiotec.2020.11.005