Nucleic Acid‐Based Microarrays and Nanostructures

Peptide nucleic acid microarrays.

A fast and economical procedure for the production of peptide nucleic acid (PNA) microarrays is presented. PNA oligomers are synthesized in a fully automatic manner in 96-well plates using standard Fmoc chemistry. Subsequently, the oligomers are released from the support and spotted onto glass or silicone slides, which were activated by succinimidyl ester. This process allows for a concomitant ...

Peptide-nucleic acid nanostructures for transfection.

Abstract To use nucleic acids in biomedical research and medical applications, these highly hydrophilic macromolecules have to be transported through the organism, targeted to specific cell surfaces, and have to cross cellular barriers. To this end, nanosized transfection complexes have been designed and several of them have been successfully tested. Here, the different steps of the transfectio...

Peptide nucleic acids on microarrays and other biosensors.

The analysis of biomolecules using microarrays and other biosensors has a significant role in molecular biotechnology, and will become even more important in the future as a versatile tool for research and diagnostics. For many applications, the synthetic DNA mimic peptide nucleic acid (PNA) could be advantageous as a probe molecule, owing to its unique physicochemical and biochemical propertie...

Analysis of nucleic acids by tandem hybridization on oligonucleotide microarrays.

Microarrays of Binary Nucleic Acid Probes for Detecting Nucleic Acid Analytes (US)