Occurrence of cassava mosaic begomoviruses in national cassava germplasm preserved in two agro‐ecological zones of Ivory Coast

Cassava production in Ivory Coast is hampered by cassava mosaic disease (CMD), which caused several begomovirus species. To increase and conserve genetic resources, the Centre National de Recherche Agronomique (CNRA) maintains a germplasm collection of 610 accessions various origins, kept open fields Bouaké Man. We carried out an epidemiological assessment molecular characterization viruses infecting CNRA germplasm. The field Man was less affected CMD (incidence 49.78%, severity 2.23, whitefly infection 2.23%), despite abundant populations (1.34 per plant), compared to 74.54%, 2.92, 8.04% population 0.34 plant). However, predominant mode transmission through infected cuttings at both sites. PCR analysis showed that East African Cameroon virus (EACMCMV) always found co-infection with (ACMV), also occurred single infection. Co-infections EACMCMV ACMV were more prevalent (52.64%) than (13.87%). Eleven complete genome sequences begomoviruses obtained, phylogenetic they are closely related isolates from Ghana, Burkina Faso Nigeria. results this study will assist breeders screen accurately for resistance progeny resulting parental lines, enabling strategic distribution new clean planting materials reduce impact CMD.