Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
نویسندگان
چکیده
منابع مشابه
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
Biological small angle X-ray scattering (BioSAXS) is a powerful technique in molecular and structural biology used to determine solution structure, particle size and shape, and surface-to-volume ratio of macromolecules. The technique is applicable to a very wide variety of solution conditions spanning a broad range of concentrations, pH values, ionic strengths, temperatures, additives, etc., bu...
متن کاملModeling of Ion-exclusion and Vacancy Ion-exclusion Chromatography on a Strongly Acidic Cation-exchange Resin in the H+ Form
Ion-exclusion chromatography (IEC) can sometimes be used for separation of the weak acids using pure water as mobile phase. Characteristic leading (i.e. frontally tailing) peaks are obtained and retention depends on the concentration of solutes. It was recently shown that this order could be reversed. In vacant ion exclusion chromatography (vIEC) sample flows as mobile phase and pure water is i...
متن کاملIon Exclusion Chromatography: Liquid Chromatography
terials, operated in a pressure-driven mode, plotted as a function of inverse temperature. This Rgure is intended to give some appreciation of the Suxes that are attainable; however, they are not normalized to a given partial pressure gradient or thickness of membrane, and thus the Suxes are not directly comparable. Taking a value of between 10 and 100 L m 2 min 1 as the level of oxygen Sux nee...
متن کاملIon-exclusion/cation-exchange chromatography (IEC/CEC) on a weakly acidic cation-exchange resin using on acidic eluent,
Ion-exclusion/cation-exchange chromatography (IEC/CEC) on a weakly acidic cation-exchange resin using on acidic eluent, developed by Tanaka et al. in 1994, has been employed to monitor the quality of environmental water samples such as acid rainwater and river water, to analyze body fluid samples such as saliva, and to evaluate water purification by photocatalytic materials.1–8 One advantage of...
متن کاملOnline ion-exchange chromatography for small-angle X-ray scattering
Biological small-angle X-ray scattering (BioSAXS) is a powerful technique to determine the solution structure, particle size, shape and surface-to-volume ratio of macromolecules. However, a drawback is that the sample needs to be monodisperse. To ensure this, size-exclusion chromatography (SEC) has been implemented on many BioSAXS beamlines. Here, the integration of ion-exchange chromatography ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2017
ISSN: 1940-087X
DOI: 10.3791/54861