Optical clearing potential of immersion-based agents applied to thick mouse brain sections
نویسندگان
چکیده
منابع مشابه
Tissue optical immersion clearing.
In this article, we discuss the optical immersion method based on refractive index matching of scatterers (e.g., collagen, elastin fibers, cells and cell compartments) and the ground material (interstitial fluid and/or cytoplasm) of tissue and blood under the action of exogenous optical clearing agents. We analyze the optical clearing of fibrous and cell-structured tissues and blood from the po...
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Optical clearing allows the reduction of light scattering in biological tissue, enabling 3D morphological information to be obtained deep within tissue using techniques such as optical projection tomography and light sheet microscopy. However, the extent of the clearing is dependent on the technique that is used. There is therefore a need for methods to quantify the quality of the clearing proc...
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Fluorescence microscopy is commonly used to investigate disease progression in biological tissues. Biological tissues, however, are strongly scattering in the visible wavelengths, limiting the application of fluorescence microscopy to superficial (<200µm) regions. Optical clearing, which involves incubation of the tissue in a chemical bath, reduces the optical scattering in tissue, resulting in...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2019
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0216064