Optical Recording of Action Potentials with Second-Harmonic Generation Microscopy
نویسندگان
چکیده
منابع مشابه
Optical recording of action potentials with second-harmonic generation microscopy.
Nonlinear microscopy has proven to be essential for neuroscience investigations of thick tissue preparations. However, the optical recording of fast (approximately 1 msec) cellular electrical activity has never until now been successfully combined with this imaging modality. Through the use of second-harmonic generation microscopy of primary Aplysia neurons in culture labeled with 4-[4-(dihexyl...
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Second-harmonic generation (SHG) has proven essential for the highest-resolution optical recording of membrane potential (Vm) in intact specimens. Here, we demonstrate single-trial SHG recordings of neuronal somatic action potentials and quantitative recordings of their decay with averaging at multiple sites during propagation along branched neurites at distances up to 350 mum from the soma. We...
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One of the main challenges in understanding the central nervous system is to measure the network dynamics of neuronal assemblies, while preserving the computational role of individual neurons. However, this is not possible with current techniques. In this work, we combined the advantages of second-harmonic generation (SHG) with a random access (RA) excitation scheme to realize a new microscope ...
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Novel scanning probe microscopy (SPM) spectroscopic techniques are currently receiving much attention. The increasing number of spectroscopic techniques, when combined with the high spatial resolution that is attainable with SPM, makes it possible to probe a large range of material characteristics on the nanoscopic scale. The ability to probe samples on the nanoscale with femtosecond time resol...
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ژورنال
عنوان ژورنال: Journal of Neuroscience
سال: 2004
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.4840-03.2004