Optimal Timing of Repeat Multiplex Molecular Testing for Respiratory Viruses
نویسندگان
چکیده
منابع مشابه
Rapid multiplex nested PCR for detection of respiratory viruses.
Respiratory tract infections can be caused by a heterogeneous group of viruses and bacteria that produce similar clinical presentations. Specific diagnosis therefore relies on laboratory investigation. This study developed and evaluated five groups of multiplex nested PCR assays that could simultaneously detect 21 different respiratory pathogens: influenza A virus (H1N1, H3N2, and H5N1); influe...
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conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...
متن کاملMicrobiology and Infectious Disease / MULTIPLEX PCR DETECTION OF RESPIRATORY VIRUSES
We evaluated the Hexaplex assay (Prodesse, Waukesha, WI) for the detection of 7 respiratory viruses (influenza A and B, parainfluenza 1-3, and respiratory syncytial virus [RSV] A and B). The Hexaplex assay was performed on 300 respiratory samples during the 1999-2000 respiratory virus season. Results of this assay were compared with shell vial cell culture and/or direct fluorescent antibody sta...
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Compared with other industrialised countries, Italy has one of the highest caesarean delivery rates, which increased from 26.1% in 1995 to 38.3% in 2005. While the overall effect of this mode of delivery on neonatal respiratory outcomes among term newborns is well known, few studies have compared the impact of gestational age at delivery on respiratory morbidity (ie, at 37 and 38 weeks’ gestati...
متن کاملDetection of respiratory viruses by molecular methods.
SUMMARY Clinical laboratories historically diagnose seven or eight respiratory virus infections using a combination of techniques including enzyme immunoassay, direct fluorescent antibody staining, cell culture, and nucleic acid amplification tests. With the discovery of six new respiratory viruses since 2000, laboratories are faced with the challenge of detecting up to 19 different viruses tha...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2020
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01203-19