Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase
نویسندگان
چکیده
منابع مشابه
Studies on oligosaccharyl transferase in yeast.
In yeast, OT consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p. Four others are not essential Ost3p, Ost4p, Ost5p, Ost6p. All yeast OT subunits have been cloned and sequenced (Kelleher et al., 1992; 2003; Kelleher & Gilmore, 1997; Kumar et a...
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Eukaryotic N-glycosylation Occurs Via Membrane-Anchored C-terminal domain of Stt3p subunit of Oligosaccharyl Transferase*
Background: Stt3p is the catalytic subunit of Oligosaccharyl Transferase (OT) that catalyses protein N-glycosylation. Results: We report the first high-resolution NMR structure of the acceptor binding domain of yeast OT. Conclusion: This work provides structural basis for the function of the C-terminal Domain of Stt3p subunit. Significance: Structure determination of this critical domain is an ...
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ژورنال
عنوان ژورنال: Protein Expression and Purification
سال: 2013
ISSN: 1046-5928
DOI: 10.1016/j.pep.2013.04.001