Osmotic adaptation of Escherichia coli with a negligible proton motive force in the presence of carbonyl cyanide m-chlorophenylhydrazone
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چکیده
منابع مشابه
proton-motive force on lactose uptake in Escherichia coli
The relationship between the steady state lactose accumulation (APia) and the magnitude of the membrane potential (Ay/) and pH gradient (ApH) has been studied at pHO5.5 and pHO7.5. An attempt has been made to differentiate between two possible means by which lactose accumulation may be reduced below the proton-motive force (Ap). Firstly, that Aqt and ApH are not equivalent in driving lactose tr...
متن کاملInteraction of carbonyl cyanide 3-chlorophenylhydrazone with cytochrome c oxidase.
Cytochrome c oxidase binds protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP) with high affinity. There are 1.46 high-affinity binding sites per cytochrome c oxidase for CCCP with dissociation constant 2.7 x 10(-7) mol/l. The bond between the CCCP and cytochrome c oxidase accomplishes through the group on cytochrome c oxidase with pKa 6.64 and is based on the electrostatic interaction...
متن کاملMutants of Mycobacterium smegmatis unable to grow at acidic pH in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone.
Mycobacterium smegmatis is able to grow and survive at acidic pH, and exhibits intracellular pH homeostasis under these conditions. In this study, the authors have identified low proton permeability of the cytoplasmic membrane, and high cytoplasmic buffering capacity, as determinants of intrinsic acid resistance of M. smegmatis. To identify genes encoding proteins involved in protecting cells f...
متن کاملActivation of Myosin Adenosine Triphosphatase by Carbonyl Cyanide P-chlorophenylhydrazone.
In an accompanying paper (l), it is shown that y-(p-arsenosophenyl)-n-butyrate activates the adenosine triphosphatase of both myosin and mitochondria, and several analogies between the properties of the active sites of these two energy transduction enzymes are discussed. Carbonyl cyanide p-chlorophenylhydrazone, which uncouples osidative phosphorylation at concentrations as low as lop7 &r (a), ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1992
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.174.9.2922-2928.1992