Osmotic Characteristics of Erythrocytes After Freezing with Composition-Modified Cryopreservatives

Here, we have studied the osmotic parameters of erythrocytes frozen in composition-modified media with glycerol and 1,2-propanediol. A decreased concentration a cryopreservative an increased content impermeable protective component sorbitol were established to affect erythrocyte characteristics at different stages freezing hypothermic storage. Reduction 1,2-propanediol NaCl concentrations when augmenting sucrose agent enabled changing two-stage mode freeze-thawing single-stage one. The presented here modification cryoprotective medium allowed diminish level damage during freeze-thawing-washing out, that might prevent inflammation development body transfusing these cells.