Outside-in Integrin Signal Transduction

Integrin signal transduction in myeloid leukocytes.

Integrin-mediated adhesion serves as a powerful costimulus for neutrophil activation. Clustering of integrins at the leukocyte membrane by interaction with surface-bound ligands (extracellular matrix proteins or endothelial cell counter-receptors) leads to a number of signaling events that culminate in actin cytoskeletal rearrangement and neutrophil functional responses such as migration, degra...

Conformational Changes in Integrin Mac - 1 Outside - In Signal Transmission

Spacing of integrin ligands influences signal transduction in endothelial cells.

The physical attributes of the extracellular matrix play a key role in endothelium function by modulating the morphology and phenotype of endothelial cells. Despite the recognized importance of matrix-cell interactions, it is currently not known how the arrangement of adhesive ligands affects the morphology, signal transduction processes, and migration of endothelial cells. We aimed to study ho...

Integrin cytoplasmic domains mediate inside-out signal transduction

We analyzed the binding of fibronectin to integrin alpha 5 beta 1 in various cells; in some cells fibronectin bound with low affinity (e.g., K562 cells) whereas in others (e.g., CHO), it bound with high affinity (Kd approximately 100 nM) in an energy-dependent manner. We constructed chimeras of the extracellular and transmembrane domains of alpha IIb beta 3 joined to the cytoplasmic domains of ...

Outside-in: peptide versus integrin.

Comment on Su et al, page 592 Outside-in: peptide versus integrin ---------------------------------------------------------------------------------------------------------------Joel S. Bennett UNIVERSITY OF PENNSYLVANIA SCHOOL OF MEDICINE In this issue of Blood, Su and colleagues report that a myristolyated cell-permeable peptide, RGT, corresponding to the extreme carboxyl terminus of the integ...