Overproduction and purification of the CcpA protein from Lactococcus lactis.
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چکیده
منابع مشابه
Overproduction and purification of the CcpA protein from Lactococcus lactis.
In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6 x His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 microg/ml ampicillin at 37 degrees C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. G...
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متن کاملIn vitro DNA binding of purified CcpA protein from Lactococcus lactis IL1403.
During this study His-tagged CcpA protein purified under native conditions to obtain a biologically active protein was used for molecular analysis of CcpA-dependent regulation. Using electrophoretic mobility shift assays it was demonstrated that CcpA of L. lactis can bind DNA in the absence of the HPr-Ser-P corepressor and exhibits DNA-binding affinity for nucleotide sequences lacking cre sites...
متن کاملRegular paper In vitro DNA binding of purified CcpA protein from Lactococcus lactis IL1403*
During this study His-tagged CcpA protein purified under native conditions to obtain a biologically active protein was used for molecular analysis of CcpA-dependent regulation. Using electrophoretic mobility shift assays it was demonstrated that CcpA of L. lactis can bind DNA in the absence of the HPr-Ser-P corepressor and exhibits DNA-binding affinity for nucleotide sequences lacking cre sites...
متن کاملPurification and characterization of an endopeptidase from Lactococcus lactis
1 Department of Chemistry and Biochemistry, Massey University, Palmerston North, New Zealand * New Zealand Dairy Research Institute, Palmerston North, New Zealand An endopeptidase has been purified from Lactococcus lactis subsp. crernoris SK11. The enzyme is a 70 kDa monomer, strongly inhibited by the metalloproteinase inhibitors 1,l O-phenanthroline and phosphoramidon but relatively insensitiv...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 2003
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.2003_3697