Oxidative Stress Boosts the Uptake of Cerium Oxide Nanoparticles by Changing Brain Endothelium Microvilli Pattern

Vascular oxidative stress is considered a worsening factor in the progression of Alzheimer’s disease (AD). Increased reactive oxygen species (ROS) levels promote accumulation amyloid-? peptide (A?), one main hallmarks AD. In turn, A? potent inducer stress. early stages AD, concomitant action and on brain capillary endothelial cells was observed to compromise blood–brain barrier functionality. this context, antioxidant compounds might provide therapeutic benefits. To aim, we investigated activity cerium oxide nanoparticles (CNP) human cerebral microvascular (hCMEC/D3) exposed oligomers. Treatment with CNP (13.9 ± 0.7 nm diameter) restored basal ROS hCMEC/D3 cells, both after acute or prolonged exposure A?. Moreover, found that extent uptake by +43% higher presence Scanning electron microscopy western blot analysis suggested changes microvilli structures cell surface, under pro-oxidant stimuli (A? H2O2), be involved enhancement uptake. This finding opens possibility exploit modulation pattern improve anti-oxidant particles designed counteract ROS-mediated cerebrovascular dysfunctions.