P-218 Does Artificial Oocyte Activation (AOA) improve the outcome beyond fertilization rate in ICSI cycles?

Abstract Study question Does CultActive© improve reproductive outcomes of ICSI cycles in cases low fertilization rate and normal rate? Summary answer The application after improves rates only clinical indication failure. What is known already Oocyte activation deficiency attributed the majority to failure cycles, these can be corrected by increasing initial levels calcium (Ca2+) using assisted oocyte techniques (AOA), such as use Ca2+ ionophores. These agents enhance intracellular release increase membrane permeability facilitating influx extracellular Ca2+, which initiates cascade. Previous results suggest that ionophore treatment live birth failed couples with poor rates, embryo developmental arrest, also number good quality embryos. design, size, duration This was a retrospective observational study. There were 30 patients included first group consisted 231 oocytes 56 embryos (without AOA). second same (n = 30) AOA, 219 47 Subgroups defined according indications (normal group: ≥ 65 % <65% ). All data collected from January-December 2022. Participants/materials, setting, methods 450 assessed belonged pre-AOA AOA. exposed for 15 minutes solution containing A23187, (Gynemed, Germany). Fertilization, blastocyst formation pregnancy analyzed.Good 3BB. Statistical significance analyzed Paired Student T test (p < 0.05 considered statistically significant). Main role chance cohort indications),was not significantly different. AOA gave 61% compared 71% control 0.1398). no blastocysts when study 0.1660). In an fertilization, higher recorded (51% 37%, respectively p 0.0238). significant difference quantity 0.3328). Regardless or there used > 0.05). Limitations, reasons caution are limitations our First, design limited population major limitations. should interpreted cautiously subgroup analyses due potential bias, specifically small without indication. Wider implications findings beneficial fertilization. Embryo did CultActiveive©. External variation minimized each cohort. suggests quality/blastulation rate. Trial registration applicable