P083 JAK/STAT pathway and IL-6 activity in moderate to severe Ulcerative Colitis

Abstract Background Ulcerative colitis (UC) is a chronic inflammatory bowel disease with high social and health care system burden. Despite recent advances in UC treatment available drugs acting different signalling pathways related to the disease, there still significant proportion of non-responders who end up being overtreated exposed treatment's secondary effects. Therefore, it would be helpful know activity each pathway involved pathogenesis order tailor best for patient. The aim our study was measure activation JAK/STAT IL-6 levels colonic biopsies from patients UC. Methods A prospective, observational single-centre designed currently on going. Adult any endoscopic (Mayo Endoscopic Score (MES) > 0) routine colonoscopy were included. Biopsies inflamed (endoscopically active) non-inflamed inactive) colon patient homogenised processed by using RIPA buffer ultrasounds obtain cell lysates. Determination performed detecting phosphorylated forms JAK1, JAK2, JAK3, TYK2, STAT1, STAT3 STAT4 Western blot. Human Luminex discovery assay used quantification. Data are shown as percentage, median, interquartile range (IQR) mean ± standard deviation appropriate. Results So far, 19 consecutively included (median age 60.9 years (IQR 40.8-66.6), 58% female). About 47% had left-sided UC, 32% extensive 21% proctitis. Regarding activity, 52.6% showed MES-3, 42.1% MES-2 5.2% MES-1. increased colon, while no found (p=0.00). Median 41.5pg/ml 7.4-106.7) 51.9pg/ml 17.5-220.5) MES-3 (p=0.4) (Figure 1). Every showed, at least, one JAK protein STAT biopsies. Nevertheless, pattern all Figure 2. Conclusion Different activated kinases detected patients, even though differs among them. not mucosa.