Pan-genomic matching statistics for targeted nanopore sequencing
نویسندگان
چکیده
منابع مشابه
Targeted enrichment of genomic DNA regions for next-generation sequencing
In this review, we discuss the latest targeted enrichment methods and aspects of their utilization along with second-generation sequencing for complex genome analysis. In doing so, we provide an overview of issues involved in detecting genetic variation, for which targeted enrichment has become a powerful tool. We explain how targeted enrichment for next-generation sequencing has made great pro...
متن کاملNanopore Sequencing: A Survey
A single molecule of Deoxyribonucleic acid (DNA) is being sequenced by using an innovative concept of Nanopore. The orientation of nanopore define the channel of ions through the pore comes into contact with nucleotide, the permutation and the transition kinematics of each molecule of DNA. The divergence of electrical signal observed when the four chemical bases adenine (A), guanine (G), cytosi...
متن کاملCas9-Assisted Targeting of CHromosome segments (CATCH) for targeted nanopore sequencing and optical genome mapping
Variations in the genetic code, from single point mutations to large structural or copy number alterations, influence susceptibility, onset, and progression of genetic diseases and tumor transformation. Next-generation sequencing analysis is unable to reliably capture aberrations larger than the typical sequencing read length of several hundred bases. Long-read, singlemolecule sequencing method...
متن کاملConversion of Genomic DNA to Proxy Constructs Suitable for Accurate Nanopore Sequencing
Nanopore sequencing at single-base resolution is challenging. There are developing technologies to convert DNA molecules to expanded constructs. Such constructs can be sequenced by nanopores in place of the original DNA molecules. We present a novel method for converting genomic DNA to expanded constructs (“proxies”) with 99.67% accuracy. Our method “reads” each base in each DNA fragment and ap...
متن کاملGenomic DNA preparation enabling multiple replicate reads for accurate nanopore sequencing
Sequencing at single-nucleotide resolution using nanopore devices is performed with reported error rates 10.5-20.7% (Ip et al., 2015). Since errors occur randomly during sequencing, repeating the sequencing procedure for the same DNA strands several times can generate sequencing results based on consensus derived from replicate readings, thus reducing overall error rates. The method presented i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: iScience
سال: 2021
ISSN: 2589-0042
DOI: 10.1016/j.isci.2021.102696