PATCH CLAMP TECHNIQUES USED FOR STUDYING SYNAPTIC TRANSMISSION IN SLICES OF MAMMALIAN BRAIN
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چکیده
منابع مشابه
Patch clamp techniques used for studying synaptic transmission in slices of mammalian brain.
Procedures are described for recording postsynaptic currents from neurones in slices of rat brain using patch clamp techniques. The method involves cutting brain slices (120-300 microns thick) with a vibrating microtome followed by localization of cell somata, which can be clearly seen with Nomarski differential interference contrast optics in the light microscope. Tissue covering the identifie...
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Brain slices from many regions of the central nervous system are an often utilized experimental system for the study of synaptic physiology and pharmacology. The hippocampal slice [ I ] has been the most highly studied of such preparations, due to its anatomically distinct synaptic pathways and disposition to synaptic plasticity [2]. The patch clamp technique [3] has permitted many new insights...
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In light of the proliferation of channels and receptors that are being cloned from the mammalian CNS it is a major challenge to understand their different roles in signalling and synaptic transmission within the brain. The patch-clamp technique developed by Neher, Sakmann and co-workers 1-3 has allowed successful studies of ionic currents and the underlying single-channel activity in the small ...
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Guanidine administration may be beneficial in the treatment of amyotrophic lateral sclerosis and related diseases; however, the actions of guanidine on the mammalian central nervous system have not been investigated. We studied the effects of this compound on neuronal properties and synaptic transmission in isolated slices of guinea pig olfactory cortex using intraand extracellular recording me...
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The effect of ketamine (1-100 µM), which has NMDA receptor antagonist properties, on synaptic transmission and long-term potentiation (LTP) in CAl area of rat hippocampus was examined in vitro. Field potentials were recorded in pyramidal cell layer following Schaffer collateral stimulation. Primed-burst stimulation (PEs) was used for induction of LTP. The amplitude of population spiks (PS) was ...
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ژورنال
عنوان ژورنال: Quarterly Journal of Experimental Physiology
سال: 1989
ISSN: 0144-8757
DOI: 10.1113/expphysiol.1989.sp003336