Pathogenicity of different rabies virus isolates and protection test in vaccinated mice

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Pathogenicity of different rabies virus isolates and protection test in vaccinated mice.

This study was aimed to evaluate and compare the pathogenicity of rabies virus isolated from bats and dogs, and to verify the efficacy of a commercial rabies vaccine against these isolates. For evaluation of pathogenicity, mice were inoculated by the intramuscular route (IM) with 500MICLD₅₀/0.03 mL of the viruses. The cross-protection test was performed by vaccinating groups of mice by the subc...

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Pathogenicity of different rabies virus variants inversely correlates with apoptosis and rabies virus glycoprotein expression in infected primary neuron cultures.

The mouse-adapted rabies virus strain CVS-24 has stable variants, CVS-B2c and CVS-N2c, which differ greatly in their pathogenicity for normal adult mice and in their ability to infect nonneuronal cells. The glycoprotein (G protein), which has previously been implicated in rabies virus pathogenicity, shows substantial structural differences between these variants. Although prior studies have ide...

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EPIDEMIOLOGICAL SURVEY OF DIFFERENT RABIES VIRUS STRAINS IN IRAN

Rabies is widespread in all provinces of Iran. This study presents an investigation by a Polymerase Chain Reaction (PCR) technique for the purpose of rabies diagnosis and also Restriction Fragment Length Polymorphism (RFLP) for determination of the type of the rabies virus. In addition, PCR proves to be a powerful tool for molecular epidemiology studies allowing analysis of the infecting strain...

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Identifying different subgroups of rabies virus in Iran

Abstract: Background: Rabies has been reported in all provinces and cities of Iran, although there has been no molecular study regarding different groups and subgroups of rabies virus by phosphoprotein gene. In this study, firstly, 48 and then 85 recent rabies isolates recovered from cases reported throughout Iran identified the evolutionary origins by molecular method of phosphoprotein gene r...

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A single immunization with recombinant rabies virus (ERAG3G) confers complete protection against rabies in mice

PURPOSE New alternative bait rabies vaccines applicable to pet dogs and wild animals are needed to eradicate rabies in Korea. In this study, recombinant rabies virus, ERAG3G strain was constructed using reverse genetic system and the safety, efficacy and immunogenicity of the ERAG3G strain was evaluated in mice and dogs. MATERIALS AND METHODS Using the full-length genome mutated amino acid at...

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ژورنال

عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo

سال: 2010

ISSN: 0036-4665

DOI: 10.1590/s0036-46652010000500002