Patterns of Protein Synthesis in T5-infected Escherichia coli

Patterns of Ribonucleic Acid Synthesis in TS-infected Escherichia coli

The role of DNA replication in the synthesis of RNA and proteins during the “late” period of the infective cycle of T!%nfected Escherichia coli F has been examined. Inhibition of DNA synthesis was achieved by addition of chloramphenicol to TS-infected cultures or by use of a mutant of T5 (T5 am I), which is unable to cause the synthesis of an active DNA polymerase upon infection of the nonpermi...

Advances and challenges in recombinant protein expression in Escherichia coli

Resistance patterns of Escherichia coli causing urinary tract infection

 Background: Urinary tract infection (UTI) is one of the most prevalent infectious diseases and Escherichia coli is its common cause. The aim of this study was to assess the resistance patterns of E.coli in urinary tract infections and to determine the susceptibility of E.coli to commonly used antimicrobials and also to evaluate the options for empirical treatment of UTI. Methods: This stud...

Synthesis of protein in a purine-requiring Escherichia coli infected with bacteriophage T2.

The Over-Expression of Biologically Active Human Growth Hormone in a T5-Based System in Escherichia coli, Studying Temperature Effect

We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...