PCR troubleshooting. The essential guide
نویسندگان
چکیده
منابع مشابه
Real-Time PCR: An Essential Guide
Chapter abstracts How to Order Real-time PCR (RT-PCR) is a powerful and rapid technique for nucleic acid amplification. The accumulation of specific products in a reaction is monitored continuously during cycling. This is usually achieved by monitoring changes in fluorescence within the PCR tube. This essential manual presents a comprehensive guide to the most appropriate and up-to-date technol...
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Department of Biological Science, Florida State University, Tallahassee, Florida 32306-3050 BASIC CONSIDERATIONS The use of PCR to generate large amounts of a desired product can be a double-edged sword. Failure to amplify a sample under optimum conditions can lead to the generation of multiple undefined and unwanted products-even to the exclusion of the desired product. At the other extreme, n...
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ژورنال
عنوان ژورنال: Expert Review of Molecular Diagnostics
سال: 2006
ISSN: 1473-7159,1744-8352
DOI: 10.1586/14737159.6.5.647