Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors
نویسندگان
چکیده
منابع مشابه
Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors
The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by ...
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It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The...
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AIMS To develop a highly sensitive and rapid protocol for simultaneous detection and differentiation of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) in pepper and tomato. In this study, we use the multiplex PCR technique to detect dual infection of these two viruses. METHODS AND RESULTS A multiplex RT-PCR method consisting of one-tube reaction with two primer pairs targeted to re...
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A new concept for the development of semiconductor field-effect biosensors by modification of a gate surface with tobacco mosaic virus (TMV) nanotubes, serving as enzyme nanocarrier, is presented. TMV nanotubes enable an immobilization of a high amount of enzymes without substantial loss of their activity, resulting in an enhanced biosensor performance. This approach has been experimentally dem...
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ژورنال
عنوان ژورنال: Nanotheranostics
سال: 2018
ISSN: 2206-7418
DOI: 10.7150/ntno.22114