Phase calibration target for quantitative phase imaging with ptychography

Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information

Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We d...

Quantitative phase contrast optimised cancerous cell differentiation via ptychography.

This paper shows that visible-light ptychography can be used to distinguish quantitatively between healthy and tumorous unstained cells. Advantages of ptychography in comparison to conventional phase-sensitive imaging techniques are highlighted. A novel procedure to automatically refocus ptychographic reconstructions is also presented, which improves quantitative analysis.

Quantitative Phase Imaging

Quantitative phase imaging with spectral-domain optical coherence phase microscopy

Spectral domain optical coherence phase microscopy provides high resolution quantitative phase measurement. Using a common path Fourier domain optical coherence tomography system, this technique is capable of excellent phase stability of less than 1 milliradian and high imaging speed of up to several hundred KHz Aline rate. The limitation of 2π ambiguity restriction can be overcome by the use o...

Quantitative phase imaging with partially coherent illumination.

In this Letter, we formulate a mathematical model for predicting experimental outcomes in quantitative phase imaging (QPI) when the illumination field is partially spatially coherent. We derive formulae that apply to QPI and discuss expected results for two classes of QPI experiments: common path and traditional interferometry, under varying degrees of spatial coherence. In particular, our resu...