Phase Separation and Protein Partitioning in Compartmentalized Cell-Free Expression Reactions

Genetically engineered charge modifications to enhance protein separation in aqueous two-phase systems: Charge directed partitioning.

This report continues or examination of the effect of genetically engineered charge modifications on the partitioning behavior of proteins in aqueous two-phase extration. The genetic modifications consisted of the fusion of charged peptide tails to beta-galactosidase and charge-change point mutations to T4 lysozyme. Our previous article examined the influence of these charge modifications on pa...

Solid-Phase Cell-Free Protein Synthesis to Improve Protein Foldability

Mitochondrial Compartmentalized Protein Folding and Tumor Cell Survival

Molecular chaperones are master regulators of protein folding quality control, and it is widely accepted that these functions are aberrantly exploited in human tumors. What has also emerged in recent years is that chaperone control of protein folding does not occur randomly in cells, but is spatially compartmentalized in subcellular organelles and specialized microenvironments. Fresh experiment...

Leishmania cell-free protein expression system.

Cell-free protein expression is an important tool for a rapid production, engineering and labeling of recombinant proteins. However the complex protocols for preparation of eukaryotic cell-free protein expression systems result in high manufacturing costs and limit their utility. Recently we reported a novel cell-free expression system based on the lysate of a fermentable protozoan Leishmania t...

Promoter-induced reactive phase separation in surface reactions.

Promoters are adsorbed mobile species which do not directly participate in a catalytic surface reaction, but can influence its rate. Often, they are characterized by strong attractive interactions with one of the reactants. We show that these conditions lead to a Turing instability of the uniform state and to the formation of reaction-induced periodic concentration patterns. Experimentally such...