Phosphopeptide detection using automated online IMAC-capillary LC-ESI-MS/MS
نویسندگان
چکیده
منابع مشابه
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Phosphopeptide identification is still a challenging task because fragmentation spectra obtained by mass spectrometry do not necessarily contain sufficient fragment ions to establish with certainty the underlying amino acid sequence and the precise phosphosite. To improve upon this, it has been suggested to acquire pairs of spectra from every phosphorylated precursor ion using different fragmen...
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Immobilized metal affinity chromatography (IMAC) using a nitrilotriacetic acid (NTA) chelating ligand functionalized with Fe(III) is regarded as one of the most selective phosphopeptide enrichment techniques for LC/MS applications. However, the use of IMAC for phosphopeptide enrichment often results in unacceptably high sample‐to‐sample and person‐to‐person variability. Phosphopeptide enrichmen...
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Immobilized metal affinity chromatography (IMAC) using a nitrilotriacetic acid (NTA) chelating ligand functionalized with Fe(III) is regarded as one of the most selective phosphopeptide enrichment techniques for LC/MS applications. However, the use of IMAC for phosphopeptide enrichment often results in unacceptably high sample‐to‐sample variability. Phosphopeptide enrichment is reliant on manua...
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ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2006
ISSN: 1615-9853,1615-9861
DOI: 10.1002/pmic.200690023