Pitfalls in drug testing by hyphenated low‐ and high‐resolution mass spectrometry
نویسندگان
چکیده
منابع مشابه
Identification of a metallothionein in Synechococcus by capillary electrophoresis hyphenated with inductively coupled plasma mass spectrometry.
A home-made system hyphenating capillary electrophoresis with an inductively coupled plasma mass spectrometer (CE-ICP-MS) for cadmium speciation of protein-binding and free cadmium ions in solution is presented. The CE-ICP-MS interface consisted of an acrylic block with an internal volume ca. 20 microL in which a platinum electrode, a capillary column, and a connection to an ICP nebulizer were ...
متن کاملProspects on the Hyphenated Electrochemistry and Mass Spectrometry as a Practical Analytical Technique in the Assessment of Oxidative Drug Metabolism
For several years, electrochemistry in combination with mass spectrometry has been the subject of attention and development for studying the oxidative drug metabolism at early stages of new drug development. Though the technique could successfully imitate the in vivo oxidative drug metabolism initiated by electron transfer, it lags in the imitation of reactions initiated by either hydrogen atom...
متن کاملPerfume Analysis by means of Fast Gas Chromatography hyphenated to Time of Flight Mass Spectrometry
Surely, as in all other industrial sectors, perfumery industries also benefit from the use of faster analyses; applied in the continuous research for new odour-active molecules as well as for quality control purposes. The GC approaches to speed up analysis include the utilization of shorter capillary columns with decreased column internal diameter and thinner stationary phase film thickness, hi...
متن کاملPitfalls in histone propionylation during bottom‐up mass spectrometry analysis
Despite their important role in regulating gene expression, posttranslational histone modifications remain technically challenging to analyze. For identification by bottom-up MS, propionylation is required prior to and following trypsin digestion. Hereby, more hydrophobic peptides are generated enabling RP HPLC separation. When histone dynamics are studied in a quantitative manner, specificity,...
متن کاملDrug metabolite profiling and identification by high-resolution mass spectrometry.
Mass spectrometry plays a key role in drug metabolite identification, an integral part of drug discovery and development. The development of high-resolution (HR) MS instrumentation with improved accuracy and stability, along with new data processing techniques, has improved the quality and productivity of metabolite identification processes. In this minireview, HR-MS-based targeted and non-targ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Drug Testing and Analysis
سال: 2020
ISSN: 1942-7603,1942-7611
DOI: 10.1002/dta.2744