Plasmid engineering

Plasmid isolation. Fatemeh Mehrpooyan*

Genetic Transformation of a Clinical (Genital Tract), Plasmid-Free Isolate of Chlamydia trachomatis: Engineering the Plasmid as a Cloning Vector

Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a replication competent plasmid) and to investigate the plasticity of the plasmid. A recently developed, plasmid-based transformation prot...

Plasmid Curing Activity of Five Plant Extracts on Multiple Resistant Plasmid Bearing Klebsiella Pnumoniae Strains

ABSTRACT The curing of five medicinal plant extracts (Satureia hortensis, Glycyrrhiza glabra, Teucrium Pollium, Myrtus Communis, Eukalypetus globolus) on plasmid bearing strains of klebsilla pnenmoniae resistance to multiple antibiotics were investigated. Alcoholic and maceration extraction were carried out from leaves, fruits or roots of the above plants. Mimimum inhibitory concentrations (MI...

Plasmid-to-plasmid recombination in Haemophilus influenzae.

No recombination between plasmids was observed after conjugal transfer of a plasmid into a cell carrying another plasmid. Two types of such recombination took place after transformation, one type being Rec+ dependent and suggesting a preferred site of recombination. The other much rarer type was at least partially Rec+ independent.

Engineering and characterization of a symbiotic selection-marker-free vector-host system for therapeutic plasmid production.

The present study aimed to develop a symbiotic selection-marker-free plasmid and host system that would allow successful plasmid maintenance and amplification for use in gene therapy. Initially, the chromosomal aspartate‑semialdehyde dehydrogenase (asd) gene was disrupted in DH10B Escherichia coli using Red recombinase‑mediated homologous recombination. This method required the use of linear DN...