Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy
نویسندگان
چکیده
منابع مشابه
Stimulated emission depletion microscopy with optical fibers
Imaging at the nanoscale and/or at remote locations holds great promise for studies in fields as disparate as the life sciences and materials sciences. One such microscopy technique, stimulated emission depletion (STED) microscopy, is one of several fluorescence based imaging techniques that offers resolution beyond the diffractionlimit. All current implementations of STED microscopy, however, ...
متن کاملSpectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores.
We report a rationale for identifying superior dyes for stimulated-emission depletion (STED) microscopy. We compared the dyes pPDI and pTDI, which displayed excellent photostability in single-molecule spectroscopy. Surprisingly, their photostability and performance in STED microscopy differed significantly. While single pTDI molecules could be visualized with excellent resolution (35 nm), pPDI ...
متن کاملDefining the Noise-limited-resolution for Stimulated Emission Depletion Microscopy
With recent developments in microscopy, such as stimulated emission deletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we analyse how the interplay between shot noise, thermal noise, the saturation intensity of a fluorescent label, and the damage threshold of the sample changes the maximum resolution of...
متن کاملDevelopment and Application of Two-Photon Excitation Stimulated Emission Depletion Microscopy for Superresolution Fluorescence Imaging in Thick Tissue
Two-photon laser scanning microscopy (2PLSM) allows fluorescence imaging in thick biological samples where absorption and scattering typically degrade resolution and signal collection of 1-photon imaging approaches. The spatial resolution of conventional 2PLSM is limited by diffraction, and the near-infrared wavelengths used for excitation in 2PLSM preclude the accurate imaging of many small su...
متن کاملStimulated Emission Depletion (STED) Microscopy: from Theory to Practice
In recent years, various methods have been proposed to increase the resolution of fluorescence microscopy beyond the diffraction limit of light. Amongst them, Stimulated Emission Depletion (STED) microscopy, which relies on a purely physical concept of fluorescence depletion could, in theory, achieve unlimited resolution independent of the wavelength of light. In this chapter, we review the fun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2017
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.06.050