Polyamine biosynthesis impacts cellular folate requirements necessary to maintain S ‐adenosylmethionine and nucleotide pools
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چکیده
منابع مشابه
Effects of polyamine biosynthesis inhibitors on S-adenosylmethionine synthet- ase and S-adenosylmethionine decarboxylase activities in carrot cell cultures
Changes in the activites o f S-adcnosylmethionine ( S A M ) synthetase (methionine adenosyltransferase, EC 2.5.1.6.) and SAM decarboxylase (EC 4.1.1.50) werc studied in carrot (Daucus carota) cell cultures in response to 2,4-dichlorophenoxyacetic acid (2,4-D) and several inhibitors o f polyamine biosynthesis. Activity o f SAM synthetase increased significantly during the first 5 days o f cultur...
متن کاملMethionine salvage and S-adenosylmethionine: essential links between sulfur, ethylene and polyamine biosynthesis.
Both Met (methionine) and SAM (S-adenosylmethionine), the activated form of Met, participate in a number of essential metabolic pathways in plants. The subcellular compartmentalization of Met fluxes will be discussed in the present review with respect to regulation and communication with the sulfur assimilation pathway, the network of the aspartate-derived amino acids and the demand for product...
متن کاملRelative effects of S-adenosylmethionine depletion on nucleic acid methylation and polyamine biosynthesis.
Treatment of cultured L1210 cells with 1 mM-L-2-amino-4-methoxy-cis-but-3-enoic acid (L-cisAMB), a methionine-analogue inhibitor of S-adenosylmethionine (AdoMet) synthetase (EC 2.5.1.6), produced a rapid and near-total depletion of AdoMet by 4 h. After this, the pools recovered to 60% of control by 48 h, apparently because of an increase in AdoMet synthetase activity. Both AdoMet depletion and ...
متن کاملModulation of polyamine-biosynthetic activity by S-adenosylmethionine depletion.
The methionine-analogue inhibitor of S-adenosylmethionine (AdoMet) synthetase, L-2-amino-4-methoxy-cis-but-3-enoic acid (L-cisAMB), was used to study the early effects of AdoMet depletion on polyamine biosynthesis. In the presence of decreased methionine (30 microM) in the medium, treatment of cultured L1210 cells with 1 mM-L-cisAMB resulted in a near-total (95%) depletion of cellular AdoMet po...
متن کاملRole of S-adenosylmethionine in methionine biosynthesis in yeast.
Extracts of Saccharomyces cerevisiae were used to develop a cell-free system capable of converting the beta-carbon of serine into the methyl group of methionine. No requirement for either S-adenosylmethionine or S-adenosylhomocysteine could be demonstrated for net methionine biosynthesis. Growth of the cells in B(12) did not affect the reaction. The mechanism for the methylation of homocysteine...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2009
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fj.09-130708