Polymer functionalization through an enzymatic process: Intermediate products characterization and their grafting onto gum Arabic

The modification of gum Arabic with ferulic acid oxidation products was performed in aqueous medium, at 30 °C and pH 7.5, the presence Myceliophthora thermophila laccase as biocatalyst. First, this study aimed to investigate structures that could possibly be covalently grafted onto Arabic. HPLC analyses revealed reaction produced several products, whose were investigated using LC-MS/MS (liquid chromatography-mass spectrometry mass fragmentation analyses) NMR experiments. chemical structure one intermediate product fully elucidated 2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl) methylidene] cyclobutane-1, 3-dione, called by authors cyclobutadiferulone. Secondly, locate grafting performing This did not determine how much specifically which but some them undeniably present modified Arabic, close glucuronic C5 carbon or galactose C6 carbon.