Polyurethane Foam/Spheroid Culture System Using Human Hepatoblastoma Cell Line (Hep G2) as a Possible New Hybrid Artificial Liver
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چکیده
منابع مشابه
Hep G2 is a hepatoblastoma-derived cell line.
[1] Brabletz T, Jung A, Spaderna S, Hlubek F, Kirchner T. Migrating cancer stem cells—an integrated concept of malignant tumor progression. Nat Rev Cancer 2005;5:744-9. [2] Carr I, Levy M, Watson P. The invasive edge: invasion in colorectal cancer. Clin Exp Metastasis 1986;4:129-39. [3] Gabbert H, Wagner R, Moll R, Gerharz CD. Tumor dedifferentiation: an important step in tumor invasion. Clin E...
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Confluent monolayers of the human hepatoblastoma-derived cell line, Hep G2, were incubated in serum-free medium. Conditioned medium was ultracentrifugally separated into d < 1.063 g/ml and d 1.063-1.20 g/ml fractions since very little VLDL was observed. The d < 1.063 g/ml fraction was examined by electron microscopy; it contained particles of 24.5 * 2.3 nm diameter, similar in size to plasma LD...
متن کاملCharacterization of lipoproteins produced by the human liver cell line, Hep G2, under defined conditions.
Confluent monolayers of the human hepatoblastoma-derived cell line, Hep G2, were incubated in serum-free medium. Conditioned medium was ultracentrifugally separated into d less than 1.063 g/ml and d 1.063-1.20 g/ml fractions since very little VLDL was observed. The d less than 1.063 g/ml fraction was examined by electron microscopy; it contained particles of 24.5 +/- 2.3 nm diameter, similar in...
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Receptor-mediated endocytosis of tissue-type plasminogen activator (t-PA) was characterized with the human hepatoma cell line Hep G2. At 4 degrees C binding of 125I-t-PA to Hep G2 cells is rapid, specific, saturable, and reflective of a homogeneous population of 76,000 high-affinity surface sites per cell (Kd = 3.7 nM). The kinetics of 125I-t-PA binding to its receptor are characterized by rate...
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The human hepatoma cell line Hep-G2 has been shown to express the major enzymes of intra- and extracellular cholesterol metabolism. These include lecithin:cholesterol acyltransferase, acyl coenzyme A:cholesterol acyltransferase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and cholesterol-7 alpha-hydroxylase. Regulatory mechanisms that have been described in other hepatic systems also appea...
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ژورنال
عنوان ژورنال: Cell Transplantation
سال: 2001
ISSN: 0963-6897,1555-3892
DOI: 10.3727/000000001783986260