Predicting Cell Wall Lytic Enzymes Using Combined Features

Ultra structural characteristics of methicillin resistant Staphylococcus aureus cell wall after affecting with lytic bacteriophages using atomic force microscopy

Objective(s): During the last years with increasing resistant bacteria to the most antibiotics bacteriophages are suggested as appropriate treatment option. To investigate lytic activity of bacteriophages there are indirect microbial procedures and direct methods. The present study to complement microbial procedures and investigate ultra-structural characteristics of infection bacterium-phage u...

Cell-wall lytic enzymes at sporulation and spore germination in Bacillus species.

When washed sporulating cells of Bacillus cereus were incubated in buffer a t 37" in the presence of toluene, a partial autolysis occurred resulting in the freeing of mature and immature spores. The autolysate contained lytic enzymes which attacked vegetative cells and cell-wall preparations, releasing hexosaminecontaining peptides of characteristic constitution. The most active enzyme preparat...

Enzymes Predicting Function using Structures

Inhibitors against Fungal Cell Wall Remodeling Enzymes.

Fungal β-1,3-glucan glucanosyltransferases are glucan-remodeling enzymes that play important roles in cell wall integrity, and are essential for the viability of pathogenic fungi and yeasts. As such, they are considered possible drug targets, although inhibitors of this class of enzymes have not yet been reported. Herein we report a multidisciplinary approach based on a structure-guided design ...

Cell wall-lytic activity in (j&Awn&Wa /wsca

The soluble fraction of homogenates of synchronous CMorg#a /w^ca was tested for carbohydrate-lyzing activities. With isolated cell walls and /?-l,4-mannan or carboxymethyl cellulose as substrates, a sharp increase in activity occurred shortly before release of the daughter cells followed by a decline during release. The lytic activities were partially purified by ammonium sulphate precipitation...