Predicting Transcriptional Output of Synthetic Multi-input Promoters

Engineering orthogonal dual transcription factors for multi-input synthetic promoters

Synthetic biology has seen an explosive growth in the capability of engineering artificial gene circuits from transcription factors (TFs), particularly in bacteria. However, most artificial networks still employ the same core set of TFs (for example LacI, TetR and cI). The TFs mostly function via repression and it is difficult to integrate multiple inputs in promoter logic. Here we present to o...

Multi - input Multi - output PI Controller Design

The design of MIMO PI controller is formulated as an LQR problem. The weighting matrices of the quadratic performance index are chosen so that tuning can be done for each inputoutput channel and for tradeoff between transient response and robustness with respect to modeling error. The number of tuning parameters is the same as that of a decentralized PI controller. A d&ign example is given to d...

Of California Santa Cruz Techniques for Synthetic Input / Output Workload

Testing Real-Time Multi Input-Output Systems

In formal testing, the assumption of input enabling is typically made. This assumption requires all inputs to be enabled anytime. In addition, the useful concept of quiescence is sometimes applied. Briefly, a system is in a quiescent state when it cannot produce outputs. In this paper, we relax the input enabling assumption, and allow some input sets to be enabled while others remain disabled. ...

Multi-stability in monotone input/output systems

This paper studies the emergence of multi-stability and hysteresis in those systems that arise, under positive feedback, starting from monotone systems with well-defined steady-state responses. Such feedback configurations appear routinely in several fields of application, and especially in biology. Characterizations of global stability behavior are stated in terms of easily checkable graphical...