Preferred conformations ofN-glycan core pentasaccharide in solution and in glycoproteins
نویسندگان
چکیده
منابع مشابه
Glycan analysis of therapeutic glycoproteins
Therapeutic monoclonal antibodies (mAbs) are glycoproteins produced by living cell systems. The glycan moieties attached to the proteins can directly affect protein stability, bioactivity, and immunogenicity. Therefore, glycan variants of a glycoprotein product must be adequately analyzed and controlled to ensure product quality. However, the inherent complexity of protein glycosylation poses a...
متن کامل‘Naked’ and Hydrated Conformers of the Conserved Core Pentasaccharide of N-linked Glycoproteins and Its Building Blocks
N-glycosylation of eukaryotic proteins is widespread and vital to survival. The pentasaccharide unit -Man3GlcNAc2- lies at the protein-junction core of all oligosaccharides attached to asparagine side chains during this process. Although its absolute conservation implies an indispensable role, associated perhaps with its structure, its unbiased conformation and the potential modulating role of ...
متن کاملmanipulation in dubbing and subtitling
پژوهش حاضر در چارچوب مکتب دستکاری قرار گرفت و با استفاده از تقسیم بندی دوکات (2007) از شیوه های دستکاری، به دنبال یافتن پاسخ برای پرسش های زیر بود: 1-رایج ترین شیوه دستکاری در دوبله فیلم ها کدام است؟ 2-رایج ترین شیوه دستکاری در زیرنویس فیلم ها کدام است؟ 3-دستکاری در دوبله فیلم ها رایج تر است یا در زیرنویس آن ها؟ این پژوهش از نوع تحقیقات توصیفی- مقایسه ای و پیکره ای می باشد. پیکره تحقیق شا...
Boiling Reduces Glycan Detection on Glycoproteins
Glycobiology is emerging as a primary field of interest for biological research. As such, it is crucial to develop and improve tools for the study of sugar-modified biomolecules. Many research endeavors in the field of protein glycobiology involve boiling of proteins in sample buffer for 5-10 minutes prior to gel electrophoresis and their further characterization. Here we examined the effect of...
متن کاملEngineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core
Yarrowia lipolytica is a dimorphic yeast that efficiently secretes various heterologous proteins and is classified as "generally recognized as safe." Therefore, it is an attractive protein production host. However, yeasts modify glycoproteins with non-human high mannose-type N-glycans. These structures reduce the protein half-life in vivo and can be immunogenic in man. Here, we describe how we ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Glycobiology
سال: 2015
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/cwv083