Preimplantation development of mouse oocytes activated by different levels of human phospholipase C zeta
نویسندگان
چکیده
منابع مشابه
Loss of activity mutations in phospholipase C zeta (PLCζ) abolishes calcium oscillatory ability of human recombinant protein in mouse oocytes.
BACKGROUND Mammalian oocyte activation occurs via a series of intracellular calcium (Ca(2+)) oscillations thought to be induced by a sperm-specific phospholipase C zeta (PLCζ). There is now strong evidence to indicate that certain types of human male infertility are caused by failure of the sperm to activate the oocyte in an appropriate manner. Molecular analysis of the PLCζ gene of a male pati...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
The Effect of Human Follicular Fluid (FF) on Preimplantation Mouse Embryo Development in Vitro
SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...
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This study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mCR2aa medium so as to obtain maximum number of embryos. A total of 1090 cumulus oocyte complexes (COC’s) were collected from 480 ovaries. In vitro matured oocytes were randomly divided into four groups. Group 1 in vitro matured oo...
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the present study sought to investigate the role of dynamic assessment (da) in improvement of iranian efl writing performance at different language proficiency levels. to this end, after conducting the quick placement test, 60 iranian efl learners were assigned to two groups with different language proficiency levels. in both groups each participant wrote two compositions, one before and one af...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2007
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/dem350