Preincubation of t-SNAREs with Complexin I Increases Content-Mixing Efficiency
نویسندگان
چکیده
منابع مشابه
Preincubation of t-SNAREs with Complexin I Increases Content-Mixing Efficiency.
Complexin (Cpx) is a major regulator for Ca(2+)-triggered fast neuroexocytosis which underlies neuronal communication. Many psychiatric and neurological disorders accompany changes in the Cpx expression level, suggesting that abnormal Cpx levels may elicit aberrant cognitive symptoms. To comprehend how the changes in the Cpx level might affect neuronal communication, we investigated Ca(2+)-trig...
متن کاملContent mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs.
Membrane bilayer fusion has been shown to be mediated by v- and t-SNAREs initially present in separate populations of liposomes and to occur with high efficiency at a physiologically meaningful rate. Lipid mixing was demonstrated to involve both the inner and the outer leaflets of the membrane bilayer. Here, we use a fusion assay that relies on duplex formation of oligonucleotides introduced in...
متن کاملComplexin cross-links prefusion SNAREs into a zigzag array
Release of neurotransmitter at the synapse must be timed precisely to immediately follow the arrival of a nervous impulse. The physiological and anatomical mechanisms for this have long been known1,2. Synaptic vesicles containing neurotransmitter are already docked at the ‘active zones’ of the presynaptic membrane, ready to respond to the elevated calcium levels that accompany an action potenti...
متن کاملi-SNAREs
A new functional class of SNAREs, designated inhibitory SNAREs (i-SNAREs), is described here. An i-SNARE inhibits fusion by substituting for or binding to a subunit of a fusogenic SNAREpin to form a nonfusogenic complex. Golgi-localized SNAREs were tested for i-SNARE activity by adding them as a fifth SNARE together with four other SNAREs that mediate Golgi fusion reactions. A striking pattern ...
متن کاملi-SNAREs: inhibitory SNAREs that fine-tune the specificity of membrane fusion
new functional class of SNAREs, designated inhibitory SNAREs (i-SNAREs), is described here. An i-SNARE inhibits fusion by substituting for or binding to a subunit of a fusogenic SNAREpin to form a nonfusogenic complex. Golgi-localized SNAREs were tested for i-SNARE activity by adding them as a fifth SNARE together with four other SNAREs that mediate Golgi fusion reactions. A striking pattern em...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2016
ISSN: 0006-2960,1520-4995
DOI: 10.1021/acs.biochem.6b00114