Primary sequence and heterologous expression of nuclear pore glycoprotein p62.
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چکیده
منابع مشابه
Primary sequence and heterologous expression of nuclear pore glycoprotein p62
The major nuclear pore protein p62 is modified by O-linked N-acetylglucosamine and functions in nuclear transport. We have cloned, sequenced, and expressed the full-length rat p62 cDNA. The rat p62 mRNA is 2,941 nucleotides long and encodes a protein of 525 amino acids containing 30% serine and threonine residues. The amino acid sequence near the amino-terminus contains unique tetrapeptide repe...
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Systemic lupus erythematosus (SLE) is a systemic autoimmune disease which is classically characterised by a variety of autoantibodies to deoxyribonucleic acid (DNA), ribonucleic acid (RNA), other nuclear and cytoplasmic antigens. Recently several novel autoantibodies against a variety of specific nuclear pore proteins have been described, including the nucleoporin p62. In this paper we evaluate...
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The complete primary structure of an integral membrane glycoprotein of the nuclear pore was deduced from the cDNA sequence. The cDNA encodes a polypeptide of 204,205 D containing a 25-residue-long signal sequence, two hydrophobic segments that could function as transmembrane segments, and 13 potential N-linked oligosaccharide addition sites. Endoglycosidase H reduces the molecular mass by appro...
متن کامل[Nuclear changes and p62 expression in ischemic and dilated cardiomyopathy].
The study's objectives were to investigate nuclear stereology and to determine the level of p62, a protein involved in nuclear transport, in human cardiomyocytes from patients with heart failure due to ischemic cardiomyopathy (ICM) or dilated cardiomyopathy (DCM). We studied 23 human hearts: 10 had ICM, 10 had DCM, and three were from control subjects. The size of the nucleus was significantly ...
متن کاملMolecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins
Macromolecular trafficking across the nuclear envelope involves interactions between cytosolic transport factors and nuclear pore complex proteins. The p62 complex, an assembly of 62, 58, 54, and 45-kD O-linked glycoproteins-localized near the central gated channel of the nuclear pore complex, has been directly implicated in nuclear protein import. The cDNA cloning of rat p62 was reported previ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1990
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.110.6.1861