Primary structure of macromomycin, an antitumor antibiotic protein.
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چکیده
منابع مشابه
Primary structure of macromomycin, an antitumor antibiotic protein.
The antitumor protein macromomycin is a single chain polypeptide of 112 amino acid residues cross-linked by two intramolecular disulfide bonds. The protein was reduced and S-alkylated with 2-mercaptoethanol in 8 M urea followed by treatment with iodoacetic acid. Tryptic digestion of tetra-S-carboxymethyl macromomycin gave four tryptic peptides which were fractionated by gel permeation on Sephad...
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Susceptibility testing by the broth dilution method showed that all the gram-positive but only some of the gram-negative bacteria tested were susceptible to the antitumor antibiotic, macromomycin (MCR; NSC 170105). The minimal inhibitory concentration for the susceptible organisms was less than 3 mug/ml. The action of MCR was bactericidal; however, at very high concentrations (50 mug/ml and abo...
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Macromomycin is a protein isolated from the culture filtrate of Streptomyces macromomyceticus. It is an antibiotic and also cytotoxic to a broad spectrum of carcinoma cells, the ID50 for P388 leukemia cells being 1 X 10(-9) M. Macromomycin binds rapidly and tightly to the P388 cell membrane and the eventual death of the cell cannot be reversed by either washing the toxin away or treating the ce...
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A numberof antimicrobial agents possess the structural unit which is a derivative of 7, 8, 9, 10-tetrahydrotetracene quinone (5, 12) present in glycosidic union with oneor more sugar units. The term anthracyclines was proposed for this group15. The recognition that one of the members, daunomycin exhibited some antitumor activity focused fresh attention on this group of compounds2). We wish to r...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)33238-1