Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties
نویسندگان
چکیده
منابع مشابه
Production, purifi cation, and characterization of α-amylase by Bacillus subtilis and its mutant derivates
Th e eff ects of various carbon and nitrogen sources on production of α-amylase by Bacillus subtilis and its mutant derivates were investigated. Th e maximum production of α-amylase by all strains was obtained in the presence of mesoinositol as the carbon source. Th ere was no more signifi cant increase in enzyme yield in the case of the supplementation of nitrogen sources, whereas malt extract...
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We produced a-amylase from banana peel by Bacillus subtilis through solid state fermentation (SSF). Addition of yeast extract, corn steep liquor, sodium dodesyl sulphate (SDS) and Tween-80 enhanced the a-amylase production under preoptimized fermentation conditions. Maximum enzyme yield (10.25 U/mL) was observed at 35°C after 24 hours of SSF in growth medium containing 0.1% yeast extract, 1% co...
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A segregated model was proposed to investigate the inherent relationships between growth, substrate consumption, cell differentiation and product formation in a Bacillus subtilis fermentation producing heterologous amylase in a 22-l bioreactor. The segregated model includes three distinguishable cell states and the transition from vegetative phase to sporangium and finally to mature spore. An a...
متن کاملBottleneck in secretion of α-amylase in Bacillus subtilis
Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In this question-oriented review, every effort is made to answer the following que...
متن کاملNOTES Partial purification and some properties of α-amylase from Bacillus subtilis KIBGE-HAS
An extracellular α-amylase from Bacillus subtilis KIBGEHAS was partially purified by ultrafiltration and ammonium sulphate precipitation with 19.2-fold purification and specific activity of 4195 U/mg. The enzyme showed relatively high thermostability and retained 62% of its activity when kept at 70°C for 15 min. α -Amylase was highly stable at −18°C and loss of activity was very low during stab...
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ژورنال
عنوان ژورنال: OALib
سال: 2021
ISSN: 2333-9721,2333-9705
DOI: 10.4236/oalib.1107291