Progress in protein engineering of Organophosphorus hydrolase (OPH)
نویسندگان
چکیده
منابع مشابه
a review on engineering of organophosphorus hydrolase (oph) enzyme
organophosphorus chemicals are compounds which have been used as pesticides and insecticides in agriculture. they’re also used as nervous agents and have raised many problems for human and environment. among the most important methods of decontamination from these compounds are biodegradation methods. using oph enzyme in degradation the mentioned compounds is seen as one of the desirable ways, ...
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Aminoalcohols have been addressed as activating buffers for alkaline phosphatase. However, there is no record on the buffer activation regarding organophosphorus hydrolase (OPH). Here we reported the activating effects of aminoalcohols on OPH-catalyzed hydrolysis of diisopropylfluorophosphate (DFP), an analog molecule of G-type warfare agents. The kinetic parametors kcat, Vmax and kcat/Km in th...
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A new anchor system based on the ice nucleation protein (InaV) from Pseudomonas syringae INA5 was developed for cell surface display of functional organophosphorus hydrolase (OPH). The activity and stability of cells expressing the truncated InaV (INPNC)-OPH fusions were compared to cells with surface-expressed OPH using two other fusion anchors based on Lpp-OmpA and the truncated InaK protein....
متن کاملEvaluation of the organophosphorus hydrolase enzyme activity in creams and investigation of its stability
The main purpose of this project is investigation of the organophosphorus hydrolase (OPH) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the OPH enzyme stability in formulated creams. OPH enzyme was extracted and purified from strain flavobacterium. The w/o and o/w creams were prepared using different formulations. In order to achieve an emulsion with m...
متن کاملDetection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase.
As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was mo...
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ژورنال
عنوان ژورنال: IOP Conference Series: Earth and Environmental Science
سال: 2021
ISSN: 1755-1315
DOI: 10.1088/1755-1315/621/1/012099