Progress on the Research Pertinent to Cultivation Constraint Factors and Improved Cultivation Methods of Environmental Uncultured Microorganisms
نویسندگان
چکیده
منابع مشابه
Laboratory cultivation of widespread and previously uncultured soil bacteria.
Most soil bacteria belong to family-level phylogenetic groups with few or no known cultivated representatives. We cultured a collection of 350 isolates from soil by using simple solid media in petri dishes. These isolates were assigned to 60 family-level groupings in nine bacterial phyla on the basis of a comparative analysis of their 16S rRNA genes. Ninety-three (27%) of the isolates belonged ...
متن کاملNew strategies for cultivation and detection of previously uncultured microbes.
An integrative approach was used to obtain pure cultures of previously uncultivated members of the divisions Acidobacteria and Verrucomicrobia from agricultural soil and from the guts of wood-feeding termites. Some elements of the cultivation procedure included the following: the use of agar media with little or no added nutrients; relatively long periods of incubation (more than 30 days); prot...
متن کاملon the comparison of keyword and semantic-context methods of learning new vocabulary meaning
the rationale behind the present study is that particular learning strategies produce more effective results when applied together. the present study tried to investigate the efficiency of the semantic-context strategy alone with a technique called, keyword method. to clarify the point, the current study seeked to find answer to the following question: are the keyword and semantic-context metho...
15 صفحه اولProgress in cultivation-independent phyllosphere microbiology
Most microorganisms of the phyllosphere are nonculturable in commonly used media and culture conditions, as are those in other natural environments. This review queries the reasons for their 'noncultivability' and assesses developments in phyllospere microbiology that have been achieved cultivation independently over the last 4 years. Analyses of total microbial communities have revealed a comp...
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ژورنال
عنوان ژورنال: Advances in Microbiology
سال: 2014
ISSN: 2327-0810,2327-0829
DOI: 10.12677/amb.2014.32003