Protein folding in a force clamp
نویسندگان
چکیده
منابع مشابه
Protein folding in a force clamp.
Kinetics of folding of a protein held in a force clamp are compared to an unconstrained folding. The comparison is made within a simple topology-based dynamical model of ubiquitin. We demonstrate that the experimentally observed variations in the end-to-end distance reflect microscopic events during folding. However, the folding scenarios in and out of the force clamp are distinct.
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Taking advantage of major improvements in their atomic force microscopy (AFM) apparatus, Fernandez and Li were able to follow single-molecule refolding trajectories of the ubiquitin protein (1). They observed a rich variety of kinetic behavior. Using a polycistronic version of ubiquitin, lengths of three to eight tethered proteins were picked up at random locations and unfolded using a pulling ...
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ژورنال
عنوان ژورنال: The Journal of Chemical Physics
سال: 2006
ISSN: 0021-9606,1089-7690
DOI: 10.1063/1.2192768