Protein freeze concentration and micro-segregation analysed in a temperature-controlled freeze container

Protein freeze concentration and micro-segregation analysed in a temperature-controlled freeze container

To examine effects of varied freezing conditions on the development of spatial heterogeneity in the frozen protein solution, macroscopic freeze concentration and micro-segregation of bovine serum albumin (BSA) were investigated in a temperature-controlled 200-ml freeze container. Freezing to -40 °C promoted formation of protein concentration gradients (69-114 μg ml-1) in frozen samples taken fr...

Freeze-drying above room temperature.

This study investigates the use of solid, organic compounds to lyophilize drugs without conventional freeze-drying equipment. The aim of the investigation is to find a pharmaceutically acceptable solvent or solvent combination that is appropriate for freeze-drying on the basis of its ability to (1) solubilize hydrophobic drugs, (2) provide a stable environment for water-sensitive compounds, (3)...

Task 2.6: Mechanical Freeze/Thaw and Freeze Concentration of Water and Wastewater Residuals

Denaturation of Enzyme Protein by Freeze-Thawing and Freeze-Drying ; I. Freeze-thawing and freeze-drying of myosin and some other muscle proteins

Changes in physico-chemical properties and m enzymatic activities of some muscle proteins by freeze-thawing or freeze-drying were measured for the purpose of clarifying the mechanism of protein denaturation by freeze-thawing and freezedrying. The results showed following: Freeze-thawing unfolded the conformation of the rod-like proteins and decreased their enzymatic activities. The two effects ...

To freeze or not to freeze

Male and female rats respond to a fearful experience in different ways, but this was not previously taken into account in research into psychiatric disorders.